Typing of Listeria monocytogenes by monocin and phage receptors
Details
Serval ID
serval:BIB_ADBB856B5803
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Typing of Listeria monocytogenes by monocin and phage receptors
Journal
International Journal of Food Microbiology
ISSN
0168-1605
Publication state
Published
Issued date
08/1996
Peer-reviewed
Oui
Volume
31
Number
1-3
Pages
245-62
Notes
Comparative Study
Journal Article --- Old month value: Aug
Journal Article --- Old month value: Aug
Abstract
One hundred strains of Listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. The monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. The results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and RAPD (random amplification of polymorphic DNA). The monocin/phage types were comparable in terms of discrimination with other methods for epidemiological investigations. The index of discrimination of using the monocin typing and phage receptor/reverse phage receptor method combination (0.99) for both the 87 epidemiologically unrelated strains and the epidemiologically important serogroup 4 strains was the highest of the seven different methods analysed. This combination of methods was simple, highly discriminatory and reproducible and can be carried out in a non-specialized laboratory. However, like most of the other Listeria typing methods, both the method and the indicator test strains need to be standardized.
Keywords
*Bacterial Typing Techniques
Bacteriocins/*classification
Bacteriophage Typing
Listeria monocytogenes/*classification
Receptors, Virus/*analysis
Reproducibility of Results
Pubmed
Web of science
Create date
11/02/2008 12:39
Last modification date
20/08/2019 15:17