Detection of aneuploid subpopulations in colon cancer biopsies by cultivation in vitro prior to flow cytometric analysis

Détails

ID Serval
serval:BIB_A7812F447272
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Detection of aneuploid subpopulations in colon cancer biopsies by cultivation in vitro prior to flow cytometric analysis
Périodique
Helvetica Chirurgica Acta
Auteur(s)
Rutz  H. P., Calmes  J. M., Burri  F., Givel  J. C., Mirimanoff  R. O.
ISSN
0018-0181 (Print)
Statut éditorial
Publié
Date de publication
08/1992
Volume
59
Numéro
2
Pages
355-8
Notes
Journal Article --- Old month value: Aug
Résumé
Flow cytometry offers a relatively simple method to determine the DNA content of colorectal cancers. However, the technique can not discover minor changes in karyotype which may characterize near-diploid tumors, nor will it detect very small populations of aneuploid cells. In an attempt to improve the detection of such cells, we incubated biopsy material prior to analysis. This method revealed an aneuploid subpopulation in one of two biopsies studied. This simple protocol may ultimately lead to a more accurate assessment of the ploidy in colon cancer.
Mots-clé
*Aneuploidy Biopsy Cell Division/genetics/physiology Colon/pathology Colonic Neoplasms/genetics/*pathology Culture Techniques DNA, Neoplasm/*genetics Flow Cytometry/*methods Humans Prognosis
Pubmed
Web of science
Création de la notice
28/01/2008 8:56
Dernière modification de la notice
20/08/2019 15:12
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