Article: article from journal or magazin.
Detection of aneuploid subpopulations in colon cancer biopsies by cultivation in vitro prior to flow cytometric analysis
Helvetica Chirurgica Acta
Journal Article --- Old month value: Aug
Flow cytometry offers a relatively simple method to determine the DNA content of colorectal cancers. However, the technique can not discover minor changes in karyotype which may characterize near-diploid tumors, nor will it detect very small populations of aneuploid cells. In an attempt to improve the detection of such cells, we incubated biopsy material prior to analysis. This method revealed an aneuploid subpopulation in one of two biopsies studied. This simple protocol may ultimately lead to a more accurate assessment of the ploidy in colon cancer.
*Aneuploidy Biopsy Cell Division/genetics/physiology Colon/pathology Colonic Neoplasms/genetics/*pathology Culture Techniques DNA, Neoplasm/*genetics Flow Cytometry/*methods Humans Prognosis
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