Identification, visualization and clonal analysis of intestinal stem cells in fish.

Détails

Ressource 1Télécharger: 3470.full.pdf (14111.11 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_A6BD66CE7E9F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification, visualization and clonal analysis of intestinal stem cells in fish.
Périodique
Development
Auteur(s)
Aghaallaei N., Gruhl F., Schaefer C.Q., Wernet T., Weinhardt V., Centanin L., Loosli F., Baumbach T., Wittbrodt J.
ISSN
1477-9129 (Electronic)
ISSN-L
0950-1991
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
143
Numéro
19
Pages
3470-3480
Langue
anglais
Résumé
Recently, a stochastic model of symmetrical stem cell division followed by neutral drift has been proposed for intestinal stem cells (ISCs), which has been suggested to represent the predominant mode of stem cell progression in mammals. In contrast, stem cells in the retina of teleost fish show an asymmetric division mode. To address whether the mode of stem cell division follows phylogenetic or ontogenetic routes, we analysed the entire gastrointestinal tract of the teleost medaka (Oryzias latipes). X-ray microcomputed tomography shows a correlation of 3D topography with the functional domains. Analysis of ISCs in proliferation assays and via genetically encoded lineage tracing highlights a stem cell niche in the furrow between the long intestinal folds that is functionally equivalent to mammalian intestinal crypts. Stem cells in this compartment are characterized by the expression of homologs of mammalian ISC markers - sox9, axin2 and lgr5 - emphasizing the evolutionary conservation of the Wnt pathway components in the stem cell niche of the intestine. The stochastic, sparse initial labelling of ISCs ultimately resulted in extended labelled or unlabelled domains originating from single stem cells in the furrow niche, contributing to both homeostasis and growth. Thus, different modes of stem cell division co-evolved within one organism, and in the absence of physical isolation in crypts, ISCs contribute to homeostatic growth.

Mots-clé
Medaka, Cell division mode, Digestive tract, Intestinal stem cells
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/03/2017 10:56
Dernière modification de la notice
20/08/2019 16:11
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