Identification, visualization and clonal analysis of intestinal stem cells in fish.

Details

Ressource 1Download: 3470.full.pdf (14111.11 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_A6BD66CE7E9F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification, visualization and clonal analysis of intestinal stem cells in fish.
Journal
Development
Author(s)
Aghaallaei N., Gruhl F., Schaefer C.Q., Wernet T., Weinhardt V., Centanin L., Loosli F., Baumbach T., Wittbrodt J.
ISSN
1477-9129 (Electronic)
ISSN-L
0950-1991
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
143
Number
19
Pages
3470-3480
Language
english
Abstract
Recently, a stochastic model of symmetrical stem cell division followed by neutral drift has been proposed for intestinal stem cells (ISCs), which has been suggested to represent the predominant mode of stem cell progression in mammals. In contrast, stem cells in the retina of teleost fish show an asymmetric division mode. To address whether the mode of stem cell division follows phylogenetic or ontogenetic routes, we analysed the entire gastrointestinal tract of the teleost medaka (Oryzias latipes). X-ray microcomputed tomography shows a correlation of 3D topography with the functional domains. Analysis of ISCs in proliferation assays and via genetically encoded lineage tracing highlights a stem cell niche in the furrow between the long intestinal folds that is functionally equivalent to mammalian intestinal crypts. Stem cells in this compartment are characterized by the expression of homologs of mammalian ISC markers - sox9, axin2 and lgr5 - emphasizing the evolutionary conservation of the Wnt pathway components in the stem cell niche of the intestine. The stochastic, sparse initial labelling of ISCs ultimately resulted in extended labelled or unlabelled domains originating from single stem cells in the furrow niche, contributing to both homeostasis and growth. Thus, different modes of stem cell division co-evolved within one organism, and in the absence of physical isolation in crypts, ISCs contribute to homeostatic growth.

Keywords
Medaka, Cell division mode, Digestive tract, Intestinal stem cells
Pubmed
Web of science
Open Access
Yes
Create date
10/03/2017 9:56
Last modification date
20/08/2019 15:11
Usage data