m6A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.

Détails

ID Serval
serval:BIB_A4D00990CF8F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
m6A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.
Périodique
Molecular cell
Auteur⸱e⸱s
Akhtar J., Renaud Y., Albrecht S., Ghavi-Helm Y., Roignant J.Y., Silies M., Junion G.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Statut éditorial
Publié
Date de publication
19/08/2021
Peer-reviewed
Oui
Volume
81
Numéro
16
Pages
3356-3367.e6
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
RNA polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m <sup>6</sup> A RNA modification regulates promoter-proximal RNAP II pausing in Drosophila cells. The m <sup>6</sup> A methyltransferase complex (MTC) and the nuclear reader Ythdc1 are recruited to gene promoters. Depleting the m <sup>6</sup> A MTC leads to a decrease in RNAP II pause release and in Ser2P occupancy on the gene body and affects nascent RNA transcription. Tethering Mettl3 to a heterologous gene promoter is sufficient to increase RNAP II pause release, an effect that relies on its m <sup>6</sup> A catalytic domain. Collectively, our data reveal an important link between RNAP II pausing and the m <sup>6</sup> A RNA modification, thus adding another layer to m <sup>6</sup> A-mediated gene regulation.
Mots-clé
Animals, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Methyltransferases/genetics, Multiprotein Complexes/genetics, Nuclear Proteins/genetics, Promoter Regions, Genetic/genetics, RNA Polymerase II/genetics, Transcription, Genetic, RNA modification, RNA polymerase II pausing, m(6)A, transcription elongation, transcriptional checkpoint
Pubmed
Web of science
Création de la notice
06/03/2020 14:53
Dernière modification de la notice
14/03/2024 7:09
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