m<sup>6</sup>A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.

Details

Serval ID
serval:BIB_A4D00990CF8F
Type
Autre: use this type when nothing else fits.
Collection
Publications
Institution
Title
m<sup>6</sup>A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.
Author(s)
Akhtar J., Renaud Y., Albrecht S., Ghavi-Helm Y., Roignant J.Y., Silies M., Junion G.
ISSN
1097-2765
ISSN-L
1097-2765
Issued date
20/07/2021
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
RNA polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m <sup>6</sup> A RNA modification regulates promoter-proximal RNAP II pausing in Drosophila cells. The m <sup>6</sup> A methyltransferase complex (MTC) and the nuclear reader Ythdc1 are recruited to gene promoters. Depleting the m <sup>6</sup> A MTC leads to a decrease in RNAP II pause release and in Ser2P occupancy on the gene body and affects nascent RNA transcription. Tethering Mettl3 to a heterologous gene promoter is sufficient to increase RNAP II pause release, an effect that relies on its m <sup>6</sup> A catalytic domain. Collectively, our data reveal an important link between RNAP II pausing and the m <sup>6</sup> A RNA modification, thus adding another layer to m <sup>6</sup> A-mediated gene regulation.
Keywords
Cell Biology, Molecular Biology, RNA modification, RNA polymerase II pausing, m(6)A, transcription elongation, transcriptional checkpoint
Pubmed
Create date
06/03/2020 15:53
Last modification date
25/07/2021 6:36
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