BACKGROUND AND OBJECTIVE: The morphological evolution of transmyocardial laser channels was analyzed in a pig model. MATERIALS AND METHODS: Five channels were created in the lateral wall of the left ventricle of 12 animals, using a Ho:YAG laser. In half of the animals, an additional infarction was induced in the same area. Animals were sacrificed at one-week intervals until week 5 and the critical regions of the left ventricular wall were subjected to microscopic computed morphometrical analysis. RESULTS: There was no clearly patent lumen at any stage. Cross-sectional area of the channels fell from 8.5 +/- 1.2 mm(2) at day 0 to 2.1 +/- 0.1 mm(2) at day 35. From day 7 onward, the channel area was gradually replaced by granulation tissue and the proportion of the channel occupied by granulation scar tissue increased from 37 +/- 2% at day 7 to 100% at day 28. In the subgroup with concomitant infarction, granulation tissue of both channel and infarction became indistinguishable from day 14 onward. CONCLUSIONS: These results suggest strongly that channel patency is not the mechanism of angina relief after transmyocardial laser revascularization with Ho:YAG laser.