Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.

Détails

ID Serval
serval:BIB_A1E23C352E2E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.
Périodique
Nucleic Acids Research
Auteur(s)
Germond J.E., ten Heggeler B., Schubiger J.L., Walker P., Westley B., Wahli W.
ISSN
0305-1048[print], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
05/1983
Volume
11
Numéro
10
Pages
2979-2997
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The isolation of the four Xenopus laevis vitellogenin genes has been completed by the purification from a DNA library of the B2 gene together with its flanking sequences. The overlapping DNA fragments analyzed cover 34 kilobases. The B2 gene which has a length of 17.5 kilobases was characterized by heteroduplex and R-loop mapping in the electron microscope and by in vitro transcription in a HeLa whole-cell extract. Its structural organization is compared with that of the closely related B1 gene. The mRNA-coding sequence of about 6 kilobases is interrupted 34 times in the B1 gene and 33 times in the B2 gene. Sequence homology between the two genes was not only found in exons. In addition, 54% of the intron sequences as well as 63% and 48.5% respectively of the 5' and 3' flanking sequences, show enough homology to form stable duplexes. These findings are compared with earlier results obtained with the two other closely related members of the vitellogenin gene family, the A1 and the A2 genes.
Mots-clé
Animals, Base Composition, Base Sequence, Cell Nucleus/metabolism, Cloning, Molecular, DNA Restriction Enzymes, Female, Genes, Hela Cells/metabolism, Humans, Lipoproteins/genetics, Liver/metabolism, Male, Microscopy, Electron, Nucleic Acid Conformation, Nucleic Acid Hybridization, RNA, Messenger/genetics, Transcription, Genetic, Vitellogenins/genetics, Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 17:05
Dernière modification de la notice
20/08/2019 16:07
Données d'usage