Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.

Details

Serval ID
serval:BIB_A1E23C352E2E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.
Journal
Nucleic Acids Research
Author(s)
Germond J.E., ten Heggeler B., Schubiger J.L., Walker P., Westley B., Wahli W.
ISSN
0305-1048[print], 0305-1048[linking]
Publication state
Published
Issued date
05/1983
Volume
11
Number
10
Pages
2979-2997
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The isolation of the four Xenopus laevis vitellogenin genes has been completed by the purification from a DNA library of the B2 gene together with its flanking sequences. The overlapping DNA fragments analyzed cover 34 kilobases. The B2 gene which has a length of 17.5 kilobases was characterized by heteroduplex and R-loop mapping in the electron microscope and by in vitro transcription in a HeLa whole-cell extract. Its structural organization is compared with that of the closely related B1 gene. The mRNA-coding sequence of about 6 kilobases is interrupted 34 times in the B1 gene and 33 times in the B2 gene. Sequence homology between the two genes was not only found in exons. In addition, 54% of the intron sequences as well as 63% and 48.5% respectively of the 5' and 3' flanking sequences, show enough homology to form stable duplexes. These findings are compared with earlier results obtained with the two other closely related members of the vitellogenin gene family, the A1 and the A2 genes.
Keywords
Animals, Base Composition, Base Sequence, Cell Nucleus/metabolism, Cloning, Molecular, DNA Restriction Enzymes, Female, Genes, Hela Cells/metabolism, Humans, Lipoproteins/genetics, Liver/metabolism, Male, Microscopy, Electron, Nucleic Acid Conformation, Nucleic Acid Hybridization, RNA, Messenger/genetics, Transcription, Genetic, Vitellogenins/genetics, Xenopus
Pubmed
Web of science
Create date
24/01/2008 16:05
Last modification date
20/08/2019 15:07
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