Embryonic stem cell-derived hematopoietic stem cells.

Détails

ID Serval
serval:BIB_A1CA4FBD806B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Embryonic stem cell-derived hematopoietic stem cells.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Wang Y., Yates F., Naveiras O., Ernst P., Daley G.Q.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
27/12/2005
Peer-reviewed
Oui
Volume
102
Numéro
52
Pages
19081-19086
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Despite two decades of studies documenting the in vitro blood-forming potential of murine embryonic stem cells (ESCs), achieving stable long-term blood engraftment of ESC-derived hematopoietic stem cells in irradiated mice has proven difficult. We have exploited the Cdx-Hox pathway, a genetic program important for blood development, to enhance the differentiation of ESCs along the hematopoietic lineage. Using an embryonic stem cell line engineered with tetracycline-inducible Cdx4, we demonstrate that ectopic Cdx4 expression promotes hematopoietic mesoderm specification, increases hematopoietic progenitor formation, and, together with HoxB4, enhances multilineage hematopoietic engraftment of lethally irradiated adult mice. Clonal analysis of retroviral integration sites confirms a common stem cell origin of lymphoid and myeloid populations in engrafted primary and secondary mice. These data document the cardinal stem cell features of self-renewal and multilineage differentiation of ESC-derived hematopoietic stem cells.
Mots-clé
Animals, Antigens, Surface/chemistry, Blotting, Southern, Cell Differentiation, Cell Line, Cell Lineage, Cell Separation, Cell Transplantation, DNA/chemistry, Embryo, Mammalian/cytology, Flow Cytometry, Gene Expression Regulation, Hematopoiesis/physiology, Hematopoietic Stem Cells/cytology, Homeodomain Proteins/metabolism, Humans, Male, Mesoderm/metabolism, Mice, Reverse Transcriptase Polymerase Chain Reaction, Spleen/cytology, Stem Cells/cytology, Time Factors, Transgenes
Pubmed
Web of science
Création de la notice
11/10/2022 0:09
Dernière modification de la notice
11/10/2022 6:39
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