Embryonic stem cell-derived hematopoietic stem cells.

Details

Serval ID
serval:BIB_A1CA4FBD806B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Embryonic stem cell-derived hematopoietic stem cells.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Wang Y., Yates F., Naveiras O., Ernst P., Daley G.Q.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
27/12/2005
Peer-reviewed
Oui
Volume
102
Number
52
Pages
19081-19086
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Despite two decades of studies documenting the in vitro blood-forming potential of murine embryonic stem cells (ESCs), achieving stable long-term blood engraftment of ESC-derived hematopoietic stem cells in irradiated mice has proven difficult. We have exploited the Cdx-Hox pathway, a genetic program important for blood development, to enhance the differentiation of ESCs along the hematopoietic lineage. Using an embryonic stem cell line engineered with tetracycline-inducible Cdx4, we demonstrate that ectopic Cdx4 expression promotes hematopoietic mesoderm specification, increases hematopoietic progenitor formation, and, together with HoxB4, enhances multilineage hematopoietic engraftment of lethally irradiated adult mice. Clonal analysis of retroviral integration sites confirms a common stem cell origin of lymphoid and myeloid populations in engrafted primary and secondary mice. These data document the cardinal stem cell features of self-renewal and multilineage differentiation of ESC-derived hematopoietic stem cells.
Keywords
Animals, Antigens, Surface/chemistry, Blotting, Southern, Cell Differentiation, Cell Line, Cell Lineage, Cell Separation, Cell Transplantation, DNA/chemistry, Embryo, Mammalian/cytology, Flow Cytometry, Gene Expression Regulation, Hematopoiesis/physiology, Hematopoietic Stem Cells/cytology, Homeodomain Proteins/metabolism, Humans, Male, Mesoderm/metabolism, Mice, Reverse Transcriptase Polymerase Chain Reaction, Spleen/cytology, Stem Cells/cytology, Time Factors, Transgenes
Pubmed
Web of science
Create date
10/10/2022 23:09
Last modification date
11/10/2022 5:39
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