In vivo survival of teicoplanin-resistant Staphylococcus aureus and fitness cost of teicoplanin resistance.

Détails

ID Serval
serval:BIB_9F50D1810F84
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo survival of teicoplanin-resistant Staphylococcus aureus and fitness cost of teicoplanin resistance.
Périodique
Antimicrobial Agents and Chemotherapy
Auteur⸱e⸱s
McCallum N., Karauzum H., Getzmann R., Bischoff M., Majcherczyk P., Berger-Bächi B., Landmann R.
ISSN
0066-4804[print], 0066-4804[linking]
Statut éditorial
Publié
Date de publication
2006
Volume
50
Numéro
7
Pages
2352-2360
Langue
anglais
Résumé
Glycopeptide resistance, in a set of in vitro step-selected teicoplanin-resistant mutants derived from susceptible Staphylococcus aureus SA113, was associated with slower growth, thickening of the bacterial cell wall, increased N-acetylglucosamine incorporation, and decreased hemolysis. Differential transcriptome analysis showed that as resistance increased, some virulence-associated genes became downregulated. In a mouse tissue cage infection model, an inoculum of 10(4) CFU of strain SA113 rapidly produced a high-bacterial-load infection, which triggered MIP-2 release, leukocyte infiltration, and reduced leukocyte viability. In contrast, with the same inoculum of the isogenic glycopeptide-resistant derivative NM67, CFU initially decreased, resulting in the elimination of the mutant in three out of seven cages. In the four cages in which NM67 survived, it partially regained wild-type characteristics, including thinning of the cell wall, reduced N-acetylglucosamine uptake, and increased hemolysis; however, the survivors also became teicoplanin hypersusceptible. The elimination of the teicoplanin-resistant mutants and selection of teicoplanin-hypersusceptible survivors in the tissue cages indicated that glycopeptide resistance imposes a fitness burden on S. aureus and is selected against in vivo, with restoration of fitness incurring the price of resistance loss.
Mots-clé
Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Drug Resistance, Bacterial, Gene Expression Regulation, Bacterial, Male, Mice, Mice, Inbred C57BL, Microbial Sensitivity Tests, Mutation, Proteome, Staphylococcal Infections/microbiology, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics, Teicoplanin/pharmacology, Virulence
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:54
Dernière modification de la notice
20/08/2019 16:05
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