In vivo survival of teicoplanin-resistant Staphylococcus aureus and fitness cost of teicoplanin resistance.

Details

Serval ID
serval:BIB_9F50D1810F84
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo survival of teicoplanin-resistant Staphylococcus aureus and fitness cost of teicoplanin resistance.
Journal
Antimicrobial Agents and Chemotherapy
Author(s)
McCallum N., Karauzum H., Getzmann R., Bischoff M., Majcherczyk P., Berger-Bächi B., Landmann R.
ISSN
0066-4804[print], 0066-4804[linking]
Publication state
Published
Issued date
2006
Volume
50
Number
7
Pages
2352-2360
Language
english
Abstract
Glycopeptide resistance, in a set of in vitro step-selected teicoplanin-resistant mutants derived from susceptible Staphylococcus aureus SA113, was associated with slower growth, thickening of the bacterial cell wall, increased N-acetylglucosamine incorporation, and decreased hemolysis. Differential transcriptome analysis showed that as resistance increased, some virulence-associated genes became downregulated. In a mouse tissue cage infection model, an inoculum of 10(4) CFU of strain SA113 rapidly produced a high-bacterial-load infection, which triggered MIP-2 release, leukocyte infiltration, and reduced leukocyte viability. In contrast, with the same inoculum of the isogenic glycopeptide-resistant derivative NM67, CFU initially decreased, resulting in the elimination of the mutant in three out of seven cages. In the four cages in which NM67 survived, it partially regained wild-type characteristics, including thinning of the cell wall, reduced N-acetylglucosamine uptake, and increased hemolysis; however, the survivors also became teicoplanin hypersusceptible. The elimination of the teicoplanin-resistant mutants and selection of teicoplanin-hypersusceptible survivors in the tissue cages indicated that glycopeptide resistance imposes a fitness burden on S. aureus and is selected against in vivo, with restoration of fitness incurring the price of resistance loss.
Keywords
Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Drug Resistance, Bacterial, Gene Expression Regulation, Bacterial, Male, Mice, Mice, Inbred C57BL, Microbial Sensitivity Tests, Mutation, Proteome, Staphylococcal Infections/microbiology, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics, Teicoplanin/pharmacology, Virulence
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:54
Last modification date
20/08/2019 15:05
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