Expression Pattern of the Pneumocystis jirovecii Major Surface Glycoprotein Superfamily in Patients with Pneumonia.

Détails

Ressource 1Télécharger: Author accepted version.pdf (1611.49 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Tous droits réservés
Document(s) secondaire(s)
Télécharger: jiaa342_suppl_supplementary_table_s1 (3).xlsx (3265.23 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_9BC913CCDA6B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression Pattern of the Pneumocystis jirovecii Major Surface Glycoprotein Superfamily in Patients with Pneumonia.
Périodique
The Journal of infectious diseases
Auteur⸱e⸱s
Schmid-Siegert E., Richard S., Luraschi A., Mühlethaler K., Pagni M., Hauser P.M.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Statut éditorial
Publié
Date de publication
03/02/2021
Peer-reviewed
Oui
Volume
223
Numéro
2
Pages
310-318
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The human pathogen Pneumocystis jirovecii harbors 6 families of major surface glycoproteins (MSGs) encoded by a single gene superfamily. MSGs are presumably responsible for antigenic variation and adhesion to host cells. The genomic organization suggests that a single member of family I is expressed at a given time per cell, whereas members of the other families are simultaneously expressed.
We analyzed RNA sequences expressed in several clinical samples, using specific weighted profiles for sorting of reads and calling of single-nucleotide variants to estimate the diversity of the expressed genes.
A number of different isoforms of at least 4 MSG families were expressed simultaneously, including isoforms of family I, for which confirmation was obtained in the wet laboratory.
These observations suggest that every single P. jirovecii population is made of individual cells with distinct surface properties. Our results enhance our understanding of the unique antigenic variation system and cell surface structure of P. jirovecii.
Mots-clé
RNAseq, colonization factor, surface antigenic variation, virulence factor
Pubmed
Web of science
Création de la notice
06/07/2020 12:31
Dernière modification de la notice
21/07/2022 6:12
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