Expression Pattern of the Pneumocystis jirovecii Major Surface Glycoprotein Superfamily in Patients with Pneumonia.

Details

Serval ID
serval:BIB_9BC913CCDA6B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression Pattern of the Pneumocystis jirovecii Major Surface Glycoprotein Superfamily in Patients with Pneumonia.
Journal
The Journal of infectious diseases
Author(s)
Schmid-Siegert E., Richard S., Luraschi A., Mühlethaler K., Pagni M., Hauser P.M.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Publication state
Published
Issued date
03/02/2021
Peer-reviewed
Oui
Volume
223
Number
2
Pages
310-318
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The human pathogen Pneumocystis jirovecii harbors 6 families of major surface glycoproteins (MSGs) encoded by a single gene superfamily. MSGs are presumably responsible for antigenic variation and adhesion to host cells. The genomic organization suggests that a single member of family I is expressed at a given time per cell, whereas members of the other families are simultaneously expressed.
We analyzed RNA sequences expressed in several clinical samples, using specific weighted profiles for sorting of reads and calling of single-nucleotide variants to estimate the diversity of the expressed genes.
A number of different isoforms of at least 4 MSG families were expressed simultaneously, including isoforms of family I, for which confirmation was obtained in the wet laboratory.
These observations suggest that every single P. jirovecii population is made of individual cells with distinct surface properties. Our results enhance our understanding of the unique antigenic variation system and cell surface structure of P. jirovecii.
Keywords
RNAseq, colonization factor, surface antigenic variation, virulence factor
Pubmed
Web of science
Create date
06/07/2020 13:31
Last modification date
07/07/2021 6:37
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