Reggies/flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_98D27A94BA19
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Reggies/flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking.
Périodique
Molecular Biology of the Cell
Auteur⸱e⸱s
Solis G.P., Schrock Y., Hülsbusch N., Wiechers M., Plattner H., Stuermer C.A.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
23
Numéro
10
Pages
1812-1825
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
The reggie/flotillin proteins are implicated in membrane trafficking and, together with the cellular prion protein (PrP), in the recruitment of E-cadherin to cell contact sites. Here, we demonstrate that reggies, as well as PrP down-regulation, in epithelial A431 cells cause overlapping processes and abnormal formation of adherens junctions (AJs). This defect in cell adhesion results from reggie effects on Src tyrosine kinases and epidermal growth factor receptor (EGFR): loss of reggies reduces Src activation and EGFR phosphorylation at residues targeted by Src and c-cbl and leads to increased surface exposure of EGFR by blocking its internalization. The prolonged EGFR signaling at the plasma membrane enhances cell motility and macropinocytosis, by which junction-associated E-cadherin is internalized and recycled back to AJs. Accordingly, blockage of EGFR signaling or macropinocytosis in reggie-deficient cells restores normal AJ formation. Thus, by promoting EGFR internalization, reggies restrict the EGFR signaling involved in E-cadherin macropinocytosis and recycling and regulate AJ formation and dynamics and thereby cell adhesion.
Mots-clé
Adherens Junctions/metabolism, Adherens Junctions/ultrastructure, Cadherins/metabolism, Cell Adhesion, Cell Line, Tumor, Cell Movement, Endocytosis, Gene Knockdown Techniques, Humans, Membrane Proteins/genetics, Membrane Proteins/metabolism, Phosphoproteins/metabolism, Phosphorylation, Prions/genetics, Prions/metabolism, Protein Processing, Post-Translational, Protein Transport, RNA Interference, Receptor, Epidermal Growth Factor/metabolism, Signal Transduction, beta Catenin/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/07/2012 15:38
Dernière modification de la notice
20/10/2020 11:12
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