Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)-carbonyl complex.

Détails

ID Serval
serval:BIB_9887E1FCA808
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)-carbonyl complex.
Périodique
Nature biotechnology
Auteur⸱e⸱s
Waibel R., Alberto R., Willuda J., Finnern R., Schibli R., Stichelberger A., Egli A., Abram U., Mach J.P., Plückthun A., Schubiger P.A.
ISSN
1087-0156
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
17
Numéro
9
Pages
897-901
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
We have developed a technetium labeling technology based on a new organometallic chemistry, which involves simple mixing of the novel reagent, a 99m Tc(I)-carbonyl compound, with a His-tagged recombinant protein. This method obviates the labeling of unpaired engineered cysteines, which frequently create problems in large-scale expression and storage of disulfide-containing proteins. In this study, we labeled antibody single-chain Fv fragments to high specific activities (90 mCi/mg), and the label was very stable to serum and all other challenges tested. The pharmacokinetic characteristics were indistinguishable from iodinated scFv fragments, and thus scFV fragments labeled by the new method will be suitable for biodistribution studies. This novel labeling method should be applicable not only to diagnostic imaging with 99mTc, but also to radioimmunotherapy approaches with 186/188 Re, and its use can be easily extended to almost any recombinant protein or synthetic peptide.
Mots-clé
Aldehydes, Animals, Antibodies/genetics, Antibodies/immunology, Chromatography, Affinity, Histidine/chemistry, Humans, Immunoglobulin Fragments/chemistry, Isotope Labeling/methods, Ketones, Mice, Mice, Nude, Mucin-1/immunology, Organotechnetium Compounds/chemical synthesis, Organotechnetium Compounds/pharmacokinetics, Radiopharmaceuticals/chemical synthesis, Radiopharmaceuticals/pharmacokinetics, Recombinant Proteins/chemistry, Recombinant Proteins/pharmacokinetics, Technetium, Tissue Distribution
Pubmed
Web of science
Création de la notice
18/11/2009 11:52
Dernière modification de la notice
20/08/2019 16:00
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