Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)-carbonyl complex.
Details
Serval ID
serval:BIB_9887E1FCA808
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)-carbonyl complex.
Journal
Nature biotechnology
ISSN
1087-0156
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
17
Number
9
Pages
897-901
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
We have developed a technetium labeling technology based on a new organometallic chemistry, which involves simple mixing of the novel reagent, a 99m Tc(I)-carbonyl compound, with a His-tagged recombinant protein. This method obviates the labeling of unpaired engineered cysteines, which frequently create problems in large-scale expression and storage of disulfide-containing proteins. In this study, we labeled antibody single-chain Fv fragments to high specific activities (90 mCi/mg), and the label was very stable to serum and all other challenges tested. The pharmacokinetic characteristics were indistinguishable from iodinated scFv fragments, and thus scFV fragments labeled by the new method will be suitable for biodistribution studies. This novel labeling method should be applicable not only to diagnostic imaging with 99mTc, but also to radioimmunotherapy approaches with 186/188 Re, and its use can be easily extended to almost any recombinant protein or synthetic peptide.
Keywords
Aldehydes, Animals, Antibodies/genetics, Antibodies/immunology, Chromatography, Affinity, Histidine/chemistry, Humans, Immunoglobulin Fragments/chemistry, Isotope Labeling/methods, Ketones, Mice, Mice, Nude, Mucin-1/immunology, Organotechnetium Compounds/chemical synthesis, Organotechnetium Compounds/pharmacokinetics, Radiopharmaceuticals/chemical synthesis, Radiopharmaceuticals/pharmacokinetics, Recombinant Proteins/chemistry, Recombinant Proteins/pharmacokinetics, Technetium, Tissue Distribution
Pubmed
Web of science
Create date
18/11/2009 11:52
Last modification date
20/08/2019 16:00