Quantitative assessment of the pool size and subset distribution of cytolytic T lymphocytes within human resting or alloactivated peripheral blood T cell populations.

Détails

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Accès restreint UNIL
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_906D1B6C4B35
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Quantitative assessment of the pool size and subset distribution of cytolytic T lymphocytes within human resting or alloactivated peripheral blood T cell populations.
Périodique
The Journal of experimental medicine
Auteur⸱e⸱s
Moretta A., Pantaleo G., Moretta L., Mingari M.C., Cerottini J.C.
ISSN
0022-1007
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
01/08/1983
Peer-reviewed
Oui
Volume
158
Numéro
2
Pages
571-585
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In order to directly assess the distribution of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) in the two major subsets of human T cells, we have used limiting dilution microculture systems to determine their frequencies. The two subsets were defined according to their reactivity (or lack thereof) with B9.4 monoclonal antibody (the specificity of which is similar, if not identical, to that of Leu 2b monoclonal antibody). Both B9+ and B9- cells obtained by sorting peripheral blood resting T cells using the fluorescence-activated cell sorter (FACS) were assayed for total CTL-P frequencies in a microculture system that allows clonal growth of every T cell. As assessed by a lectin-dependent assay, approximately 30% of peripheral blood T cells were CTP-P. In the B9+ subset (which represents 20-30% of all T cells), the CTL-P frequency was close to 100%, whereas the B9- subset had a 25-fold lower CTL-P frequency. It is thus evident that 90% and 10% of the total CTL-P in peripheral blood are confined to the B9+ or B9- T cell subsets, respectively. Analysis of the subset distribution of CTL-P directed against a given set of alloantigens confirmed these findings. CTL-P frequencies were also determined in B9+ and B9- subsets derived from T cells that had been activated in allogenic mixed leucocyte cultures (MLC). Approximately 10% of MLC T cells were CTL-P. This frequency was increased 3.5-fold in the B9+ subset, whereas the B9- subset contained only a small, although detectable number of CTL-P. Moreover, the great majority of the (operationally defined) CTL-P in MLC T cell population were found to be directed against the stimulating alloantigens, thus indicating a dramatic increase in specific CTL-P frequencies following in vitro stimulation in bulk cultures.
Mots-clé
Antibodies, Monoclonal/immunology, Antigens, Differentiation, T-Lymphocyte, Antigens, Surface/analysis, Clone Cells/immunology, Cytotoxicity Tests, Immunologic, Hematopoietic Stem Cells/classification, Hematopoietic Stem Cells/immunology, Humans, Leukocyte Count, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/classification, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
09/08/2024 15:52
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