Quantitative assessment of the pool size and subset distribution of cytolytic T lymphocytes within human resting or alloactivated peripheral blood T cell populations

Details

Serval ID
serval:BIB_906D1B6C4B35
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantitative assessment of the pool size and subset distribution of cytolytic T lymphocytes within human resting or alloactivated peripheral blood T cell populations
Journal
Journal of Experimental Medicine
Author(s)
Moretta  A., Pantaleo  G., Moretta  L., Mingari  M. C., Cerottini  J. C.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
08/1983
Volume
158
Number
2
Pages
571-85
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Abstract
In order to directly assess the distribution of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) in the two major subsets of human T cells, we have used limiting dilution microculture systems to determine their frequencies. The two subsets were defined according to their reactivity (or lack thereof) with B9.4 monoclonal antibody (the specificity of which is similar, if not identical, to that of Leu 2b monoclonal antibody). Both B9+ and B9- cells obtained by sorting peripheral blood resting T cells using the fluorescence-activated cell sorter (FACS) were assayed for total CTL-P frequencies in a microculture system that allows clonal growth of every T cell. As assessed by a lectin-dependent assay, approximately 30% of peripheral blood T cells were CTP-P. In the B9+ subset (which represents 20-30% of all T cells), the CTL-P frequency was close to 100%, whereas the B9- subset had a 25-fold lower CTL-P frequency. It is thus evident that 90% and 10% of the total CTL-P in peripheral blood are confined to the B9+ or B9- T cell subsets, respectively. Analysis of the subset distribution of CTL-P directed against a given set of alloantigens confirmed these findings. CTL-P frequencies were also determined in B9+ and B9- subsets derived from T cells that had been activated in allogenic mixed leucocyte cultures (MLC). Approximately 10% of MLC T cells were CTL-P. This frequency was increased 3.5-fold in the B9+ subset, whereas the B9- subset contained only a small, although detectable number of CTL-P. Moreover, the great majority of the (operationally defined) CTL-P in MLC T cell population were found to be directed against the stimulating alloantigens, thus indicating a dramatic increase in specific CTL-P frequencies following in vitro stimulation in bulk cultures.
Keywords
Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte Antigens, Surface/analysis Clone Cells/immunology Cytotoxicity Tests, Immunologic Hematopoietic Stem Cells/classification/immunology Humans Leukocyte Count *Lymphocyte Activation Lymphocyte Culture Test, Mixed T-Lymphocytes/immunology T-Lymphocytes, Cytotoxic/classification/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:14
Last modification date
20/08/2019 15:53
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