Cryo-electron microscopy of vitrified insect flight muscle

Détails

ID Serval
serval:BIB_8DE98BB179CF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cryo-electron microscopy of vitrified insect flight muscle
Périodique
Journal of Molecular Biology
Auteur(s)
McDowall  A. W., Hofmann  W., Lepault  J., Adrian  M., Dubochet  J.
ISSN
0022-2836 (Print)
Statut éditorial
Publié
Date de publication
09/1984
Volume
178
Numéro
1
Pages
105-11
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 5
Résumé
Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy. High resolution images with good contrast were obtained. As judged from direct images and from the comparison of optical diffractograms with X-ray diffraction patterns obtained from glycerinated muscle fibres, the vitrified specimen is well preserved. Preparation artifacts encountered in conventional plastic sections are avoided. The preservation of the relaxed structure also seems better than in freeze-etched replicas as the 14.5 nm repeat of crossbridges on thick filaments is easily visible.
Mots-clé
Animals Flight, Animal Freezing Insects/*ultrastructure Microscopy, Electron Muscle Contraction Muscle Relaxation Muscles/*ultrastructure Preservation, Biological/methods X-Ray Diffraction
Pubmed
Web of science
Création de la notice
24/01/2008 10:25
Dernière modification de la notice
20/08/2019 14:51
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