Cryo-electron microscopy of vitrified insect flight muscle

Details

Serval ID
serval:BIB_8DE98BB179CF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryo-electron microscopy of vitrified insect flight muscle
Journal
Journal of Molecular Biology
Author(s)
McDowall  A. W., Hofmann  W., Lepault  J., Adrian  M., Dubochet  J.
ISSN
0022-2836 (Print)
Publication state
Published
Issued date
09/1984
Volume
178
Number
1
Pages
105-11
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 5
Abstract
Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy. High resolution images with good contrast were obtained. As judged from direct images and from the comparison of optical diffractograms with X-ray diffraction patterns obtained from glycerinated muscle fibres, the vitrified specimen is well preserved. Preparation artifacts encountered in conventional plastic sections are avoided. The preservation of the relaxed structure also seems better than in freeze-etched replicas as the 14.5 nm repeat of crossbridges on thick filaments is easily visible.
Keywords
Animals Flight, Animal Freezing Insects/*ultrastructure Microscopy, Electron Muscle Contraction Muscle Relaxation Muscles/*ultrastructure Preservation, Biological/methods X-Ray Diffraction
Pubmed
Web of science
Create date
24/01/2008 10:25
Last modification date
20/08/2019 14:51
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