Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.

Détails

ID Serval
serval:BIB_8D96EF9801E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.
Périodique
FASEB journal
Auteur(s)
Ives A., Le Roy D., Théroude C., Bernhagen J., Roger T., Calandra T.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Statut éditorial
Publié
Date de publication
05/2021
Peer-reviewed
Oui
Volume
35
Numéro
5
Pages
e21418
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Constitutively expressed by innate immune cells, the cytokine macrophage migration inhibitory factor (MIF) initiates host immune responses and drives pathogenic responses in infectious, inflammatory, and autoimmune diseases. Dendritic cells (DCs) express high levels of MIF, but the role of MIF in DC function remains poorly characterized. As migration is critical for DC immune surveillance, we investigated whether MIF promoted the migration of DCs. In classical transwell experiments, MIF <sup>-/-</sup> bone marrow-derived DCs (BMDCs) or MIF <sup>+/+</sup> BMDCs treated with ISO-1, an inhibitor of MIF, showed markedly reduced spontaneous migration and chemotaxis. CD74 <sup>-/-</sup> BMDCs that are deficient in the ligand-binding component of the cognate MIF receptor exhibited a migration defect similar to that of MIF <sup>-/-</sup> BMDCs. Adoptive transfer experiments of LPS-matured MIF <sup>+/+</sup> and MIF <sup>-/-</sup> and of CD74 <sup>+/+</sup> and CD74 <sup>-/-</sup> BMDCs injected into the hind footpads of homologous or heterologous mice showed that the autocrine and paracrine MIF activity acting via CD74 contributed to the recruitment of DCs to the draining lymph nodes. Mechanistically, MIF activated the Src/PI3K signaling pathway and myosin II complexes, which were required for the migration of BMDCs. Altogether, these data show that the cytokine MIF exerts chemokine-like activity for DC motility and trafficking.
Mots-clé
MIF, chemokine, cytokine, dendritic cell, innate immunity, motility
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/04/2021 14:56
Dernière modification de la notice
12/06/2021 6:33
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