Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_8D48181EEDFD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein
Périodique
PLoS Biology
Auteur(s)
Rosso L., Marques A. C., Weier M., Lambert N., Lambot M. A., Vanderhaeghen P., Kaessmann H.
ISSN
1545-7885
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
6
Numéro
6
Pages
e140
Langue
anglais
Résumé
Gene duplication was prevalent during hominoid evolution, yet little is known about the functional fate of new ape gene copies. We characterized the CDC14B cell cycle gene and the functional evolution of its hominoid-specific daughter gene, CDC14Bretro. We found that CDC14B encodes four different splice isoforms that show different subcellular localizations (nucleus or microtubule-associated) and functional properties. A microtubular CDC14B variant spawned CDC14Bretro through retroposition in the hominoid ancestor 18-25 million years ago (Mya). CDC14Bretro evolved brain-/testis-specific expression after the duplication event and experienced a short period of intense positive selection in the African ape ancestor 7-12 Mya. Using resurrected ancestral protein variants, we demonstrate that by virtue of amino acid substitutions in distinct protein regions during this time, the subcellular localization of CDC14Bretro progressively shifted from the association with microtubules (stabilizing them) to an association with the endoplasmic reticulum. CDC14Bretro evolution represents a paradigm example of rapid, selectively driven subcellular relocalization, thus revealing a novel mode for the emergence of new gene function
Mots-clé
Amino Acid Sequence , Amino Acid Substitution , analysis , Animals , Brain , Cell Cycle Proteins , Cell Line , Dual-Specificity Phosphatases , Evolution,Molecular , Gene Duplication , Genes,Duplicate , genetics , Genomics , Hominidae , Humans , metabolism , Molecular Sequence Data , physiology , Protein Isoforms , Proteins , Switzerland
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2009 23:14
Dernière modification de la notice
20/08/2019 15:51
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