Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein

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Serval ID
serval:BIB_8D48181EEDFD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein
Journal
PLoS Biology
Author(s)
Rosso L., Marques A. C., Weier M., Lambert N., Lambot M. A., Vanderhaeghen P., Kaessmann H.
ISSN
1545-7885
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
6
Number
6
Pages
e140
Language
english
Abstract
Gene duplication was prevalent during hominoid evolution, yet little is known about the functional fate of new ape gene copies. We characterized the CDC14B cell cycle gene and the functional evolution of its hominoid-specific daughter gene, CDC14Bretro. We found that CDC14B encodes four different splice isoforms that show different subcellular localizations (nucleus or microtubule-associated) and functional properties. A microtubular CDC14B variant spawned CDC14Bretro through retroposition in the hominoid ancestor 18-25 million years ago (Mya). CDC14Bretro evolved brain-/testis-specific expression after the duplication event and experienced a short period of intense positive selection in the African ape ancestor 7-12 Mya. Using resurrected ancestral protein variants, we demonstrate that by virtue of amino acid substitutions in distinct protein regions during this time, the subcellular localization of CDC14Bretro progressively shifted from the association with microtubules (stabilizing them) to an association with the endoplasmic reticulum. CDC14Bretro evolution represents a paradigm example of rapid, selectively driven subcellular relocalization, thus revealing a novel mode for the emergence of new gene function
Keywords
Amino Acid Sequence , Amino Acid Substitution , analysis , Animals , Brain , Cell Cycle Proteins , Cell Line , Dual-Specificity Phosphatases , Evolution,Molecular , Gene Duplication , Genes,Duplicate , genetics , Genomics , Hominidae , Humans , metabolism , Molecular Sequence Data , physiology , Protein Isoforms , Proteins , Switzerland
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2009 22:14
Last modification date
20/08/2019 14:51
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