Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging.

Détails

ID Serval
serval:BIB_895958E3046E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging.
Périodique
Lab on a Chip
Auteur(s)
Robinson T., Schaerli Y., Wootton R., Hollfelder F., Dunsby C., Baldwin G., Neil M., French P., deMello A.
ISSN
1473-0189 (Electronic)
ISSN-L
1473-0189
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
9
Numéro
23
Pages
3437-3441
Langue
anglais
Résumé
We report a method for removing unwanted contributions to fluorescence signals from dyes absorbed in polydimethylsiloxane (PDMS) using fluorescence lifetime imaging microscopy (FLIM). By analysing experimental fluorescence decays using a bi-exponential decay model, we are able to discriminate between emission originating from dye molecules in free solution and those absorbed within the PDMS substrate. Simple image processing allows the unwanted background signal to be removed and thus enables a more accurate assessment of temperature. The efficacy of the approach is demonstrated by measuring temperature changes within a droplet-based PCR device.

Mots-clé
Adsorption, Dimethylpolysiloxanes/chemistry, Equipment Design, Fluorescence, Fluorescent Dyes/analysis, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Microscopy, Confocal/methods, Rhodamines/analysis, Temperature
Pubmed
Web of science
Création de la notice
02/02/2017 8:14
Dernière modification de la notice
20/08/2019 14:48
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