Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging.

Details

Serval ID
serval:BIB_895958E3046E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging.
Journal
Lab on a Chip
Author(s)
Robinson T., Schaerli Y., Wootton R., Hollfelder F., Dunsby C., Baldwin G., Neil M., French P., deMello A.
ISSN
1473-0189 (Electronic)
ISSN-L
1473-0189
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
9
Number
23
Pages
3437-3441
Language
english
Abstract
We report a method for removing unwanted contributions to fluorescence signals from dyes absorbed in polydimethylsiloxane (PDMS) using fluorescence lifetime imaging microscopy (FLIM). By analysing experimental fluorescence decays using a bi-exponential decay model, we are able to discriminate between emission originating from dye molecules in free solution and those absorbed within the PDMS substrate. Simple image processing allows the unwanted background signal to be removed and thus enables a more accurate assessment of temperature. The efficacy of the approach is demonstrated by measuring temperature changes within a droplet-based PCR device.

Keywords
Adsorption, Dimethylpolysiloxanes/chemistry, Equipment Design, Fluorescence, Fluorescent Dyes/analysis, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Microscopy, Confocal/methods, Rhodamines/analysis, Temperature
Pubmed
Web of science
Create date
02/02/2017 8:14
Last modification date
20/08/2019 14:48
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