Efficient T cell activation requires an optimal dwell-time of interaction between the TCR and the pMHC complex.

Détails

ID Serval
serval:BIB_890D638689DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Efficient T cell activation requires an optimal dwell-time of interaction between the TCR and the pMHC complex.
Périodique
Nature immunology
Auteur⸱e⸱s
Kalergis A.M., Boucheron N., Doucey M.A., Palmieri E., Goyarts E.C., Vegh Z., Luescher I.F., Nathenson S.G.
ISSN
1529-2908
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
2
Numéro
3
Pages
229-234
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Cytotoxic T cell (CTL) activation by antigen requires the specific detection of peptide-major histocompatibility class I (pMHC) molecules on the target-cell surface by the T cell receptor (TCR). We examined the effect of mutations in the antigen-binding site of a Kb-restricted TCR on T cell activation, antigen binding and dissociation from antigen.These parameters were also examined for variants derived from a Kd-restricted peptide that was recognized by a CTL clone. Using these two independent systems, we show that T cell activation can be impaired by mutations that either decrease or increase the binding half-life of the TCR-pMHC interaction. Our data indicate that efficient T cell activation occurs within an optimal dwell-time range of TCR-pMHC interaction. This restricted dwell-time range is consistent with the exclusion of either extremely low or high affinity T cells from the expanded population during immune responses.
Mots-clé
Animals, Antigens/genetics, Antigens/immunology, Cytokines/biosynthesis, Half-Life, Histocompatibility Antigens Class I/genetics, Histocompatibility Antigens Class I/immunology, Hybridomas, Kinetics, Lymphocyte Activation, Mutagenesis, Site-Directed, Nucleocapsid/genetics, Nucleocapsid/immunology, Nucleocapsid Proteins, Peptides/genetics, Peptides/immunology, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 11:20
Dernière modification de la notice
20/08/2019 14:48
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