Efficient T cell activation requires an optimal dwell-time of interaction between the TCR and the pMHC complex.
Details
Serval ID
serval:BIB_890D638689DC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Efficient T cell activation requires an optimal dwell-time of interaction between the TCR and the pMHC complex.
Journal
Nature immunology
ISSN
1529-2908
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
2
Number
3
Pages
229-234
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
Cytotoxic T cell (CTL) activation by antigen requires the specific detection of peptide-major histocompatibility class I (pMHC) molecules on the target-cell surface by the T cell receptor (TCR). We examined the effect of mutations in the antigen-binding site of a Kb-restricted TCR on T cell activation, antigen binding and dissociation from antigen.These parameters were also examined for variants derived from a Kd-restricted peptide that was recognized by a CTL clone. Using these two independent systems, we show that T cell activation can be impaired by mutations that either decrease or increase the binding half-life of the TCR-pMHC interaction. Our data indicate that efficient T cell activation occurs within an optimal dwell-time range of TCR-pMHC interaction. This restricted dwell-time range is consistent with the exclusion of either extremely low or high affinity T cells from the expanded population during immune responses.
Keywords
Animals, Antigens/genetics, Antigens/immunology, Cytokines/biosynthesis, Half-Life, Histocompatibility Antigens Class I/genetics, Histocompatibility Antigens Class I/immunology, Hybridomas, Kinetics, Lymphocyte Activation, Mutagenesis, Site-Directed, Nucleocapsid/genetics, Nucleocapsid/immunology, Nucleocapsid Proteins, Peptides/genetics, Peptides/immunology, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Create date
28/01/2008 11:20
Last modification date
20/08/2019 14:48