Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes

Détails

ID Serval
serval:BIB_887EF23E82A0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes
Périodique
Journal of Experimental Medicine
Auteur⸱e⸱s
Romero  P., Dunbar  P. R., Valmori  D., Pittet  M., Ogg  G. S., Rimoldi  D., Chen  J. L., Lienard  D., Cerottini  J. C., Cerundolo  V.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
11/1998
Volume
188
Numéro
9
Pages
1641-50
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 2
Résumé
Characterization of cytolytic T lymphocyte (CTL) responses to tumor antigens has been impeded by a lack of direct assays of CTL activity. We have synthesized reagents ("tetramers") that specifically stain CTLs recognizing melanoma antigens. Tetramer staining of tumor-infiltrated lymph nodes ex vivo revealed high frequencies of tumor-specific CTLs which were antigen-experienced by surface phenotype. In vitro culture of lymph node cells with cytokines resulted in very large expansions of tumor-specific CTLs that were dependent on the presence of tumor cells in the lymph nodes. Tetramer-guided sorting by flow cytometer allowed isolation of melanoma-specific CTLs and confirmation of their specificity and their ability to lyse autologous tumor cells. Our results demonstrate the value of these novel reagents for monitoring tumor-specific CTL responses and for generating CTLs for adoptive immunotherapy. These data also indicate that strong CTL responses to melanoma often occur in vivo, and that the reactive CTLs have substantial proliferative and tumoricidal potential.
Mots-clé
Adult Aged Amino Acid Sequence Antigens, Neoplasm/genetics/*metabolism Female Histocompatibility Antigens Class I/chemistry/*metabolism Humans Immunotherapy, Adoptive Indicators and Reagents Lymphatic Metastasis/*immunology/*pathology Lymphocytes, Tumor-Infiltrating/*immunology/*pathology Male Melanoma/*immunology/*secondary/therapy Middle Aged Monophenol Monooxygenase/genetics/immunology Neoplasm Proteins/genetics/immunology Peptide Fragments/genetics/immunology Protein Conformation Staining and Labeling T-Lymphocytes, Cytotoxic/*immunology/*pathology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:13
Dernière modification de la notice
20/08/2019 14:47
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