Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes
Details
Serval ID
serval:BIB_887EF23E82A0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes
Journal
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
11/1998
Volume
188
Number
9
Pages
1641-50
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 2
Research Support, Non-U.S. Gov't --- Old month value: Nov 2
Abstract
Characterization of cytolytic T lymphocyte (CTL) responses to tumor antigens has been impeded by a lack of direct assays of CTL activity. We have synthesized reagents ("tetramers") that specifically stain CTLs recognizing melanoma antigens. Tetramer staining of tumor-infiltrated lymph nodes ex vivo revealed high frequencies of tumor-specific CTLs which were antigen-experienced by surface phenotype. In vitro culture of lymph node cells with cytokines resulted in very large expansions of tumor-specific CTLs that were dependent on the presence of tumor cells in the lymph nodes. Tetramer-guided sorting by flow cytometer allowed isolation of melanoma-specific CTLs and confirmation of their specificity and their ability to lyse autologous tumor cells. Our results demonstrate the value of these novel reagents for monitoring tumor-specific CTL responses and for generating CTLs for adoptive immunotherapy. These data also indicate that strong CTL responses to melanoma often occur in vivo, and that the reactive CTLs have substantial proliferative and tumoricidal potential.
Keywords
Adult
Aged
Amino Acid Sequence
Antigens, Neoplasm/genetics/*metabolism
Female
Histocompatibility Antigens Class I/chemistry/*metabolism
Humans
Immunotherapy, Adoptive
Indicators and Reagents
Lymphatic Metastasis/*immunology/*pathology
Lymphocytes, Tumor-Infiltrating/*immunology/*pathology
Male
Melanoma/*immunology/*secondary/therapy
Middle Aged
Monophenol Monooxygenase/genetics/immunology
Neoplasm Proteins/genetics/immunology
Peptide Fragments/genetics/immunology
Protein Conformation
Staining and Labeling
T-Lymphocytes, Cytotoxic/*immunology/*pathology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
20/08/2019 15:47