Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes.
Details
Download: 9802976_BIB_887EF23E82A0.pdf (158.74 [Ko])
State: Public
Version: Final published version
License: CC BY-NC-SA 4.0
State: Public
Version: Final published version
License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_887EF23E82A0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes.
Journal
The Journal of experimental medicine
ISSN
0022-1007
ISSN-L
0022-1007
Publication state
Published
Issued date
02/11/1998
Peer-reviewed
Oui
Volume
188
Number
9
Pages
1641-1650
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Characterization of cytolytic T lymphocyte (CTL) responses to tumor antigens has been impeded by a lack of direct assays of CTL activity. We have synthesized reagents ("tetramers") that specifically stain CTLs recognizing melanoma antigens. Tetramer staining of tumor-infiltrated lymph nodes ex vivo revealed high frequencies of tumor-specific CTLs which were antigen-experienced by surface phenotype. In vitro culture of lymph node cells with cytokines resulted in very large expansions of tumor-specific CTLs that were dependent on the presence of tumor cells in the lymph nodes. Tetramer-guided sorting by flow cytometer allowed isolation of melanoma-specific CTLs and confirmation of their specificity and their ability to lyse autologous tumor cells. Our results demonstrate the value of these novel reagents for monitoring tumor-specific CTL responses and for generating CTLs for adoptive immunotherapy. These data also indicate that strong CTL responses to melanoma often occur in vivo, and that the reactive CTLs have substantial proliferative and tumoricidal potential.
Keywords
Adult, Aged, Amino Acid Sequence, Antigens, Neoplasm/genetics, Antigens, Neoplasm/metabolism, Female, Histocompatibility Antigens Class I/chemistry, Histocompatibility Antigens Class I/metabolism, Humans, Immunotherapy, Adoptive, Indicators and Reagents, Lymphatic Metastasis/immunology, Lymphatic Metastasis/pathology, Lymphocytes, Tumor-Infiltrating/immunology, Lymphocytes, Tumor-Infiltrating/pathology, MART-1 Antigen, Male, Melanoma/immunology, Melanoma/secondary, Melanoma/therapy, Middle Aged, Monophenol Monooxygenase/genetics, Monophenol Monooxygenase/immunology, Neoplasm Proteins/genetics, Neoplasm Proteins/immunology, Peptide Fragments/genetics, Peptide Fragments/immunology, Protein Conformation, Staining and Labeling, T-Lymphocytes, Cytotoxic/immunology, T-Lymphocytes, Cytotoxic/pathology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:13
Last modification date
09/08/2024 15:02