Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.

Détails

ID Serval
serval:BIB_88136C9E54A3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.
Périodique
Methods in Molecular Biology
Auteur(s)
Horigome C., Dion V., Seeber A., Gehlen L.R., Gasser S.M.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
1292
Pages
77-96
Langue
anglais
Résumé
Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.
Pubmed
Web of science
Création de la notice
29/06/2015 11:24
Dernière modification de la notice
20/08/2019 14:47
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