Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.

Details

Serval ID
serval:BIB_88136C9E54A3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.
Journal
Methods in Molecular Biology
Author(s)
Horigome C., Dion V., Seeber A., Gehlen L.R., Gasser S.M.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
1292
Pages
77-96
Language
english
Abstract
Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.
Pubmed
Web of science
Create date
29/06/2015 11:24
Last modification date
20/08/2019 14:47
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