A standardized toolkit for genetic engineering of CTG clade yeasts.

Détails

ID Serval
serval:BIB_86F8089ABB9C
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
A standardized toolkit for genetic engineering of CTG clade yeasts.
Périodique
Journal of microbiological methods
Auteur⸱e⸱s
Defosse T.A., Courdavault V., Coste A.T., Clastre M., de Bernonville T.D., Godon C., Vandeputte P., Lanoue A., Touzé A., Linder T., Droby S., Rosa C.A., Sanglard D., d'Enfert C., Bouchara J.P., Giglioli-Guivarc'h N., Papon N.
ISSN
1872-8359 (Electronic)
ISSN-L
0167-7012
Statut éditorial
Publié
Date de publication
01/2018
Peer-reviewed
Oui
Volume
144
Pages
152-156
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Mots-clé
Codon, Fluorescence, Gene Expression Regulation, Fungal, Genetic Engineering/methods, Genetic Engineering/standards, Green Fluorescent Proteins, Luciferases, Luminescent Measurements/methods, Luminescent Measurements/standards, Molecular Biology/methods, Staining and Labeling, Transformation, Genetic, Yeasts/genetics, beta-Galactosidase, Candida, Fluorescent proteins, Luciferase, Standardization, Synthetic construct, β-galactosidase
Pubmed
Web of science
Création de la notice
23/11/2017 20:22
Dernière modification de la notice
20/08/2019 15:46
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