A standardized toolkit for genetic engineering of CTG clade yeasts.
Détails
ID Serval
serval:BIB_86F8089ABB9C
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
A standardized toolkit for genetic engineering of CTG clade yeasts.
Périodique
Journal of microbiological methods
ISSN
1872-8359 (Electronic)
ISSN-L
0167-7012
Statut éditorial
Publié
Date de publication
01/2018
Peer-reviewed
Oui
Volume
144
Pages
152-156
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Mots-clé
Codon, Fluorescence, Gene Expression Regulation, Fungal, Genetic Engineering/methods, Genetic Engineering/standards, Green Fluorescent Proteins, Luciferases, Luminescent Measurements/methods, Luminescent Measurements/standards, Molecular Biology/methods, Staining and Labeling, Transformation, Genetic, Yeasts/genetics, beta-Galactosidase, Candida, Fluorescent proteins, Luciferase, Standardization, Synthetic construct, β-galactosidase
Pubmed
Web of science
Création de la notice
23/11/2017 19:22
Dernière modification de la notice
20/08/2019 14:46