A standardized toolkit for genetic engineering of CTG clade yeasts.
Details
Serval ID
serval:BIB_86F8089ABB9C
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
A standardized toolkit for genetic engineering of CTG clade yeasts.
Journal
Journal of microbiological methods
ISSN
1872-8359 (Electronic)
ISSN-L
0167-7012
Publication state
Published
Issued date
01/2018
Peer-reviewed
Oui
Volume
144
Pages
152-156
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Keywords
Codon, Fluorescence, Gene Expression Regulation, Fungal, Genetic Engineering/methods, Genetic Engineering/standards, Green Fluorescent Proteins, Luciferases, Luminescent Measurements/methods, Luminescent Measurements/standards, Molecular Biology/methods, Staining and Labeling, Transformation, Genetic, Yeasts/genetics, beta-Galactosidase, Candida, Fluorescent proteins, Luciferase, Standardization, Synthetic construct, β-galactosidase
Pubmed
Web of science
Create date
23/11/2017 20:22
Last modification date
20/08/2019 15:46