A standardized toolkit for genetic engineering of CTG clade yeasts.

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Serval ID
serval:BIB_86F8089ABB9C
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
UNIL/CHUV
Title
A standardized toolkit for genetic engineering of CTG clade yeasts.
Journal
Journal of microbiological methods
Author(s)
Defosse T.A., Courdavault V., Coste A.T., Clastre M., de Bernonville T.D., Godon C., Vandeputte P., Lanoue A., Touzé A., Linder T., Droby S., Rosa C.A., Sanglard D., d'Enfert C., Bouchara J.P., Giglioli-Guivarc'h N., Papon N.
ISSN
1872-8359 (Electronic)
ISSN-L
0167-7012
Publication state
Published
Issued date
01/2018
Peer-reviewed
Oui
Volume
144
Pages
152-156
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Keywords
Codon, Fluorescence, Gene Expression Regulation, Fungal, Genetic Engineering/methods, Genetic Engineering/standards, Green Fluorescent Proteins, Luciferases, Luminescent Measurements/methods, Luminescent Measurements/standards, Molecular Biology/methods, Staining and Labeling, Transformation, Genetic, Yeasts/genetics, beta-Galactosidase, Candida, Fluorescent proteins, Luciferase, Standardization, Synthetic construct, β-galactosidase
OAI-PMH
oai:serval.unil.ch:BIB_86F8089ABB9C
DOI
10.1016/j.mimet.2017.11.015
Pubmed
29155237
Web of science
000424176200026
Create date
23/11/2017 20:22
Last modification date
20/08/2019 15:46
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