A human omentum-specific mesothelial-like stromal population inhibits adipogenesis through IGFBP2 secretion.
Détails
Télécharger: 38729152.pdf (10244.73 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_852F74DC799C
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
A human omentum-specific mesothelial-like stromal population inhibits adipogenesis through IGFBP2 secretion.
Périodique
Cell metabolism
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Statut éditorial
Publié
Date de publication
02/07/2024
Peer-reviewed
Oui
Volume
36
Numéro
7
Pages
1566-1585.e9
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Adipose tissue plasticity is orchestrated by molecularly and functionally diverse cells within the stromal vascular fraction (SVF). Although several mouse and human adipose SVF cellular subpopulations have by now been identified, we still lack an understanding of the cellular and functional variability of adipose stem and progenitor cell (ASPC) populations across human fat depots. To address this, we performed single-cell and bulk RNA sequencing (RNA-seq) analyses of >30 SVF/Lin- samples across four human adipose depots, revealing two ubiquitous human ASPC (hASPC) subpopulations with distinct proliferative and adipogenic properties but also depot- and BMI-dependent proportions. Furthermore, we identified an omental-specific, high IGFBP2-expressing stromal population that transitions between mesothelial and mesenchymal cell states and inhibits hASPC adipogenesis through IGFBP2 secretion. Our analyses highlight the molecular and cellular uniqueness of different adipose niches, while our discovery of an anti-adipogenic IGFBP2+ omental-specific population provides a new rationale for the biomedically relevant, limited adipogenic capacity of omental hASPCs.
Mots-clé
Humans, Adipogenesis, Omentum/metabolism, Omentum/cytology, Insulin-Like Growth Factor Binding Protein 2/metabolism, Insulin-Like Growth Factor Binding Protein 2/genetics, Stromal Cells/metabolism, Stromal Cells/cytology, Female, Male, Middle Aged, Adipose Tissue/metabolism, Adipose Tissue/cytology, Adult, Epithelium/metabolism, Stem Cells/metabolism, Stem Cells/cytology, Mesenchymal Stem Cells/metabolism, Mesenchymal Stem Cells/cytology, Aged, Animals, Human adipose tissue, IGFBP2, adipogenesis, adipose stem and progenitor cells, anti-adipogenic, mesothelial cells, mesothelial to mesenchymal transition, omentum, scRNA-seq, visceral adipose tissue
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/05/2024 8:17
Dernière modification de la notice
26/07/2024 6:13