Gene Expression Profiling of Cutaneous Injured and Non-Injured Nociceptors in SNI Animal Model of Neuropathic Pain.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_8491E723675C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene Expression Profiling of Cutaneous Injured and Non-Injured Nociceptors in SNI Animal Model of Neuropathic Pain.
Périodique
Scientific reports
Auteur(s)
Berta T., Perrin F.E., Pertin M., Tonello R., Liu Y.C., Chamessian A., Kato A.C., Ji R.R., Decosterd I.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
24/08/2017
Peer-reviewed
Oui
Volume
7
Numéro
1
Pages
9367
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Nociceptors are a particular subtype of dorsal root ganglion (DRG) neurons that detect noxious stimuli and elicit pain. Although recent efforts have been made to reveal the molecular profile of nociceptors in normal conditions, little is known about how this profile changes in pathological conditions. In this study we exploited laser capture microdissection to specifically collect individual injured and non-injured nociceptive DRG neurons and to define their gene profiling in rat spared nerve injury (SNI) model of neuropathic pain. We found minimal transcriptional changes in non-injured neurons at 7 days after SNI. In contrast, several novel transcripts were altered in injured nociceptors, and the global signature of these LCM-captured neurons differed markedly from that the gene expression patterns found previously using whole DRG tissue following SNI. Pathway analysis of the transcriptomic profile of the injured nociceptors revealed oxidative stress as a key biological process. We validated the increase of caspase-6 (CASP6) in small-sized DRG neurons and its functional role in SNI- and paclitaxel-induced neuropathic pain. Our results demonstrate that the identification of gene regulation in a specific population of DRG neurons (e.g., nociceptors) is an effective strategy to reveal new mechanisms and therapeutic targets for neuropathic pain from different origins.
Mots-clé
Animals, Biopsy, Caspase 6/metabolism, Computational Biology, Disease Models, Animal, Ganglia, Spinal, Gene Expression Profiling, Humans, Immunohistochemistry, Mice, Mice, Knockout, Neuralgia/etiology, Neuralgia/metabolism, Neuralgia/pathology, Nociceptors/metabolism, Nociceptors/pathology, Paclitaxel/adverse effects, Rats, Skin/injuries, Spinal Nerves/injuries, Transcriptome
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/09/2017 17:20
Dernière modification de la notice
30/04/2021 6:12
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