Gene Expression Profiling of Cutaneous Injured and Non-Injured Nociceptors in SNI Animal Model of Neuropathic Pain.

Details

Ressource 1Download: 28839165_BIB_8491E723675C.pdf (4334.09 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_8491E723675C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Gene Expression Profiling of Cutaneous Injured and Non-Injured Nociceptors in SNI Animal Model of Neuropathic Pain.
Journal
Scientific reports
Author(s)
Berta T., Perrin F.E., Pertin M., Tonello R., Liu Y.C., Chamessian A., Kato A.C., Ji R.R., Decosterd I.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
24/08/2017
Peer-reviewed
Oui
Volume
7
Number
1
Pages
9367
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Nociceptors are a particular subtype of dorsal root ganglion (DRG) neurons that detect noxious stimuli and elicit pain. Although recent efforts have been made to reveal the molecular profile of nociceptors in normal conditions, little is known about how this profile changes in pathological conditions. In this study we exploited laser capture microdissection to specifically collect individual injured and non-injured nociceptive DRG neurons and to define their gene profiling in rat spared nerve injury (SNI) model of neuropathic pain. We found minimal transcriptional changes in non-injured neurons at 7 days after SNI. In contrast, several novel transcripts were altered in injured nociceptors, and the global signature of these LCM-captured neurons differed markedly from that the gene expression patterns found previously using whole DRG tissue following SNI. Pathway analysis of the transcriptomic profile of the injured nociceptors revealed oxidative stress as a key biological process. We validated the increase of caspase-6 (CASP6) in small-sized DRG neurons and its functional role in SNI- and paclitaxel-induced neuropathic pain. Our results demonstrate that the identification of gene regulation in a specific population of DRG neurons (e.g., nociceptors) is an effective strategy to reveal new mechanisms and therapeutic targets for neuropathic pain from different origins.
Keywords
Animals, Biopsy, Caspase 6/metabolism, Computational Biology, Disease Models, Animal, Ganglia, Spinal, Gene Expression Profiling, Humans, Immunohistochemistry, Mice, Mice, Knockout, Neuralgia/etiology, Neuralgia/metabolism, Neuralgia/pathology, Nociceptors/metabolism, Nociceptors/pathology, Paclitaxel/adverse effects, Rats, Skin/injuries, Spinal Nerves/injuries, Transcriptome
Pubmed
Web of science
Open Access
Yes
Create date
25/09/2017 17:20
Last modification date
30/04/2021 6:12
Usage data