Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Wuggenig, P.; Kaya, B.; Melhem, H.; Ayata, C.K.; Hruz, P.; Sayan, A.E.; Tsumura, H.; Ito, M.; Roux, J.; Niess, J.H.

doi:10.1038/s42003-020-0842-3

Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Détails

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Accès restreint UNIL
Etat: Public
Version: de l'auteur⸱e
Licence: CC BY 4.0

ID Serval

serval:BIB_814786699E95

Type

Article: article d'un périodique ou d'un magazine.

Collection

Publications

Institution

UNIL/CHUV

Titre

Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Périodique

Communications biology

Auteur⸱e⸱s

Wuggenig P., Kaya B., Melhem H., Ayata C.K., Hruz P., Sayan A.E., Tsumura H., Ito M., Roux J., Niess J.H.

Collaborateur⸱rice⸱s

Swiss IBD Cohort Investigators

Contributeur⸱rice⸱s

Abdelrahman K., Ademi G., Aepli P., Anderegg C., Antonino A.T., Archanioti E., Arrigoni E., de Jong D.B., Balsiger B., Bastürk P., Bauerfeind P., Becocci A., Belli D., Bengoa J.M., Biedermann L., Binek J., Blattmann M., Boehm S., Boldanova T., Borovicka J., Braegger C.P., Brand S., Brügger L., Brunner S., Bühr P., Burk S., Burnand B., Burri E., Buyse S., Cao D.T., Carstens O., Criblez D.H., Cunningham S., D'Angelo F., de Saussure P., Degen L., Delarive J., Doerig C., Dora B., Drerup S., Egger M., El-Wafa A., Engelmann M., Ezri J., Felley C., Fliegner M., Fournier N., Fraga M., Franc Y., Frei R., Frei P., Fried M., Froehlich F., Furlano R.I., Garzoni L., Geyer M., Girard L., Girardin M., Golay D., Good I., Bigler U.G., Gysi B., Haarer J., Halama M., Haldemann J., Heer P., Heimgartner B., Helbling B., Hengstler P., Herzog D., Hess C., Hessler R., Heyland K., Hinterleitner T., Hirschi C., Hruz P., Juillerat P., Kayser S., Keller C., Khalid-de Bakker C., Knellwolf-Grieger C., Knoblauch C., Köhler H., Koller R., Krieger-Grübel C., Künzler P., Kusche R., Lehmann F.S., Macpherson A.J., Maillard M.H., Manz M., Marot A., Meier R., Meyenberger C., Meyer P., Michetti P., Misselwitz B., Mosler P., Mottet C., Müller C., Müllhaupt B., Musso L., Neagu M., Nichita C., Niess J.H., Nydegger A., Obialo N., Ollo D., Oropesa C., Peter U., Peternac D., Petit L.M., Pittet V., Pohl D., Porzner M., Preissler C., Raschle N., Rentsch R., Restellini S., Restellini A., Richterich J.P., Ris F., Risti B., Ritz M.A., Rogler G., Röhrich N., Rossel J.B., Rueger V., Rusticeanu M., Sagmeister M., Saner G., Sauter B., Sawatzki M., Scharl M., Schelling M., Schibli S., Schlauri H., Schluckebier D., Schmid-Uebelhart S., Schmid D., Schnegg J.F., Schoepfer A., Seematter V., Seibold F., Seirafi M., Semadeni G.M., Senning A., Sokollik C., Sommer J., Spalinger J., Spangenberger H., Stadler P., Staub P., Staudenmann D., Stenz V., Steuerwald M., Straumann A., Strebel B., Stulz A., Sulz M., Tatu A., Tempia-Caliera M., Thomas A., Thorens J., Truninger K., Tutuian R., Urfer P., Vavricka S., Viani F., Vögtlin J., Von Känel R., Vouillamoz D., Vulliamy R., Wiesel P., Wiest R., Wöhrle S., Wylie T., Zamora S., Zander S., Zeitz J., Zimmermann D.

ISSN

2399-3642 (Electronic)

ISSN-L

2399-3642

Statut éditorial

Publié

Date de publication

18/03/2020

Peer-reviewed

Oui

Volume

Numéro

Pages

130

Langue

anglais

Notes

Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish

Résumé

Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid transporter CD98hc in CX3CR1 <sup>+</sup> macrophages was generated. The relatively selective deletion of CD98hc in macrophage populations leads to attenuated severity of chemically-induced colitis that we assessed by clinical, endoscopic, and histological scoring. Single-cell RNA sequencing of colonic lamina propria macrophages revealed that conditional deletion of CD98hc alters the "monocyte waterfall"-development to MHC II <sup>+</sup> macrophages. The change in the macrophage development after deletion of CD98hc is associated with increased apoptotic gene expression. Our results show that CD98hc deletion changes the development of colonic macrophages.

Mots-clé

Adolescent, Adult, Aged, Aged, 80 and over, Amino Acids, Branched-Chain/metabolism, Animals, Apoptosis, Apoptosis Regulatory Proteins/genetics, Apoptosis Regulatory Proteins/metabolism, CX3C Chemokine Receptor 1/genetics, CX3C Chemokine Receptor 1/metabolism, Colitis/chemically induced, Colitis/metabolism, Colitis/pathology, Colitis/prevention & control, Colitis, Ulcerative/diagnosis, Colitis, Ulcerative/genetics, Colitis, Ulcerative/metabolism, Colon/metabolism, Colon/ultrastructure, Crohn Disease/diagnosis, Crohn Disease/genetics, Crohn Disease/metabolism, Dextran Sulfate, Disease Models, Animal, Female, Fusion Regulatory Protein 1, Heavy Chain/deficiency, Fusion Regulatory Protein 1, Heavy Chain/genetics, Gene Expression Regulation, Humans, Intestinal Mucosa/metabolism, Intestinal Mucosa/ultrastructure, Macrophages/metabolism, Macrophages/ultrastructure, Male, Mice, Inbred C57BL, Mice, Knockout, Middle Aged, Phenotype, RNA-Seq, Single-Cell Analysis, Young Adult

OAI-PMH

oai:serval.unil.ch:BIB_814786699E95

DOI

10.1038/s42003-020-0842-3

Pubmed

32188932

Web of science

000522135700001

Open Access

Oui

Création de la notice

20/07/2021 11:26