Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Wuggenig, P.; Kaya, B.; Melhem, H.; Ayata, C.K.; Hruz, P.; Sayan, A.E.; Tsumura, H.; Ito, M.; Roux, J.; Niess, J.H.

doi:10.1038/s42003-020-0842-3

Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Details

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State: Public
Version: author
License: CC BY 4.0

Serval ID

serval:BIB_814786699E95

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Journal

Communications biology

Author(s)

Wuggenig P., Kaya B., Melhem H., Ayata C.K., Hruz P., Sayan A.E., Tsumura H., Ito M., Roux J., Niess J.H.

Working group(s)

Swiss IBD Cohort Investigators

Contributor(s)

Abdelrahman K., Ademi G., Aepli P., Anderegg C., Antonino A.T., Archanioti E., Arrigoni E., de Jong D.B., Balsiger B., Bastürk P., Bauerfeind P., Becocci A., Belli D., Bengoa J.M., Biedermann L., Binek J., Blattmann M., Boehm S., Boldanova T., Borovicka J., Braegger C.P., Brand S., Brügger L., Brunner S., Bühr P., Burk S., Burnand B., Burri E., Buyse S., Cao D.T., Carstens O., Criblez D.H., Cunningham S., D'Angelo F., de Saussure P., Degen L., Delarive J., Doerig C., Dora B., Drerup S., Egger M., El-Wafa A., Engelmann M., Ezri J., Felley C., Fliegner M., Fournier N., Fraga M., Franc Y., Frei R., Frei P., Fried M., Froehlich F., Furlano R.I., Garzoni L., Geyer M., Girard L., Girardin M., Golay D., Good I., Bigler U.G., Gysi B., Haarer J., Halama M., Haldemann J., Heer P., Heimgartner B., Helbling B., Hengstler P., Herzog D., Hess C., Hessler R., Heyland K., Hinterleitner T., Hirschi C., Hruz P., Juillerat P., Kayser S., Keller C., Khalid-de Bakker C., Knellwolf-Grieger C., Knoblauch C., Köhler H., Koller R., Krieger-Grübel C., Künzler P., Kusche R., Lehmann F.S., Macpherson A.J., Maillard M.H., Manz M., Marot A., Meier R., Meyenberger C., Meyer P., Michetti P., Misselwitz B., Mosler P., Mottet C., Müller C., Müllhaupt B., Musso L., Neagu M., Nichita C., Niess J.H., Nydegger A., Obialo N., Ollo D., Oropesa C., Peter U., Peternac D., Petit L.M., Pittet V., Pohl D., Porzner M., Preissler C., Raschle N., Rentsch R., Restellini S., Restellini A., Richterich J.P., Ris F., Risti B., Ritz M.A., Rogler G., Röhrich N., Rossel J.B., Rueger V., Rusticeanu M., Sagmeister M., Saner G., Sauter B., Sawatzki M., Scharl M., Schelling M., Schibli S., Schlauri H., Schluckebier D., Schmid-Uebelhart S., Schmid D., Schnegg J.F., Schoepfer A., Seematter V., Seibold F., Seirafi M., Semadeni G.M., Senning A., Sokollik C., Sommer J., Spalinger J., Spangenberger H., Stadler P., Staub P., Staudenmann D., Stenz V., Steuerwald M., Straumann A., Strebel B., Stulz A., Sulz M., Tatu A., Tempia-Caliera M., Thomas A., Thorens J., Truninger K., Tutuian R., Urfer P., Vavricka S., Viani F., Vögtlin J., Von Känel R., Vouillamoz D., Vulliamy R., Wiesel P., Wiest R., Wöhrle S., Wylie T., Zamora S., Zander S., Zeitz J., Zimmermann D.

ISSN

2399-3642 (Electronic)

ISSN-L

2399-3642

Publication state

Published

Issued date

18/03/2020

Peer-reviewed

Oui

Volume

Number

Pages

130

Language

english

Notes

Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish

Abstract

Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid transporter CD98hc in CX3CR1 <sup>+</sup> macrophages was generated. The relatively selective deletion of CD98hc in macrophage populations leads to attenuated severity of chemically-induced colitis that we assessed by clinical, endoscopic, and histological scoring. Single-cell RNA sequencing of colonic lamina propria macrophages revealed that conditional deletion of CD98hc alters the "monocyte waterfall"-development to MHC II <sup>+</sup> macrophages. The change in the macrophage development after deletion of CD98hc is associated with increased apoptotic gene expression. Our results show that CD98hc deletion changes the development of colonic macrophages.

Keywords

Adolescent, Adult, Aged, Aged, 80 and over, Amino Acids, Branched-Chain/metabolism, Animals, Apoptosis, Apoptosis Regulatory Proteins/genetics, Apoptosis Regulatory Proteins/metabolism, CX3C Chemokine Receptor 1/genetics, CX3C Chemokine Receptor 1/metabolism, Colitis/chemically induced, Colitis/metabolism, Colitis/pathology, Colitis/prevention & control, Colitis, Ulcerative/diagnosis, Colitis, Ulcerative/genetics, Colitis, Ulcerative/metabolism, Colon/metabolism, Colon/ultrastructure, Crohn Disease/diagnosis, Crohn Disease/genetics, Crohn Disease/metabolism, Dextran Sulfate, Disease Models, Animal, Female, Fusion Regulatory Protein 1, Heavy Chain/deficiency, Fusion Regulatory Protein 1, Heavy Chain/genetics, Gene Expression Regulation, Humans, Intestinal Mucosa/metabolism, Intestinal Mucosa/ultrastructure, Macrophages/metabolism, Macrophages/ultrastructure, Male, Mice, Inbred C57BL, Mice, Knockout, Middle Aged, Phenotype, RNA-Seq, Single-Cell Analysis, Young Adult

OAI-PMH

oai:serval.unil.ch:BIB_814786699E95

DOI

10.1038/s42003-020-0842-3

Pubmed

32188932

Web of science

000522135700001

Open Access

Yes

Create date

20/07/2021 11:26