Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.

Details

Serval ID
serval:BIB_814786699E95
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice.
Journal
Communications biology
Author(s)
Wuggenig P., Kaya B., Melhem H., Ayata C.K., Hruz P., Sayan A.E., Tsumura H., Ito M., Roux J., Niess J.H.
Working group(s)
Swiss IBD Cohort Investigators
Contributor(s)
Abdelrahman K., Ademi G., Aepli P., Anderegg C., Antonino A.T., Archanioti E., Arrigoni E., de Jong D.B., Balsiger B., Bastürk P., Bauerfeind P., Becocci A., Belli D., Bengoa J.M., Biedermann L., Binek J., Blattmann M., Boehm S., Boldanova T., Borovicka J., Braegger C.P., Brand S., Brügger L., Brunner S., Bühr P., Burk S., Burnand B., Burri E., Buyse S., Cao D.T., Carstens O., Criblez D.H., Cunningham S., D'Angelo F., de Saussure P., Degen L., Delarive J., Doerig C., Dora B., Drerup S., Egger M., El-Wafa A., Engelmann M., Ezri J., Felley C., Fliegner M., Fournier N., Fraga M., Franc Y., Frei R., Frei P., Fried M., Froehlich F., Furlano R.I., Garzoni L., Geyer M., Girard L., Girardin M., Golay D., Good I., Bigler U.G., Gysi B., Haarer J., Halama M., Haldemann J., Heer P., Heimgartner B., Helbling B., Hengstler P., Herzog D., Hess C., Hessler R., Heyland K., Hinterleitner T., Hirschi C., Hruz P., Juillerat P., Kayser S., Keller C., Khalid-de Bakker C., Knellwolf-Grieger C., Knoblauch C., Köhler H., Koller R., Krieger-Grübel C., Künzler P., Kusche R., Lehmann F.S., Macpherson A.J., Maillard M.H., Manz M., Marot A., Meier R., Meyenberger C., Meyer P., Michetti P., Misselwitz B., Mosler P., Mottet C., Müller C., Müllhaupt B., Musso L., Neagu M., Nichita C., Niess J.H., Nydegger A., Obialo N., Ollo D., Oropesa C., Peter U., Peternac D., Petit L.M., Pittet V., Pohl D., Porzner M., Preissler C., Raschle N., Rentsch R., Restellini S., Restellini A., Richterich J.P., Ris F., Risti B., Ritz M.A., Rogler G., Röhrich N., Rossel J.B., Rueger V., Rusticeanu M., Sagmeister M., Saner G., Sauter B., Sawatzki M., Scharl M., Schelling M., Schibli S., Schlauri H., Schluckebier D., Schmid-Uebelhart S., Schmid D., Schnegg J.F., Schoepfer A., Seematter V., Seibold F., Seirafi M., Semadeni G.M., Senning A., Sokollik C., Sommer J., Spalinger J., Spangenberger H., Stadler P., Staub P., Staudenmann D., Stenz V., Steuerwald M., Straumann A., Strebel B., Stulz A., Sulz M., Tatu A., Tempia-Caliera M., Thomas A., Thorens J., Truninger K., Tutuian R., Urfer P., Vavricka S., Viani F., Vögtlin J., Von Känel R., Vouillamoz D., Vulliamy R., Wiesel P., Wiest R., Wöhrle S., Wylie T., Zamora S., Zander S., Zeitz J., Zimmermann D.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Publication state
Published
Issued date
18/03/2020
Peer-reviewed
Oui
Volume
3
Number
1
Pages
130
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid transporter CD98hc in CX3CR1 <sup>+</sup> macrophages was generated. The relatively selective deletion of CD98hc in macrophage populations leads to attenuated severity of chemically-induced colitis that we assessed by clinical, endoscopic, and histological scoring. Single-cell RNA sequencing of colonic lamina propria macrophages revealed that conditional deletion of CD98hc alters the "monocyte waterfall"-development to MHC II <sup>+</sup> macrophages. The change in the macrophage development after deletion of CD98hc is associated with increased apoptotic gene expression. Our results show that CD98hc deletion changes the development of colonic macrophages.
Keywords
Adolescent, Adult, Aged, Aged, 80 and over, Amino Acids, Branched-Chain/metabolism, Animals, Apoptosis, Apoptosis Regulatory Proteins/genetics, Apoptosis Regulatory Proteins/metabolism, CX3C Chemokine Receptor 1/genetics, CX3C Chemokine Receptor 1/metabolism, Colitis/chemically induced, Colitis/metabolism, Colitis/pathology, Colitis/prevention & control, Colitis, Ulcerative/diagnosis, Colitis, Ulcerative/genetics, Colitis, Ulcerative/metabolism, Colon/metabolism, Colon/ultrastructure, Crohn Disease/diagnosis, Crohn Disease/genetics, Crohn Disease/metabolism, Dextran Sulfate, Disease Models, Animal, Female, Fusion Regulatory Protein 1, Heavy Chain/deficiency, Fusion Regulatory Protein 1, Heavy Chain/genetics, Gene Expression Regulation, Humans, Intestinal Mucosa/metabolism, Intestinal Mucosa/ultrastructure, Macrophages/metabolism, Macrophages/ultrastructure, Male, Mice, Inbred C57BL, Mice, Knockout, Middle Aged, Phenotype, RNA-Seq, Single-Cell Analysis, Young Adult
Pubmed
Web of science
Open Access
Yes
Create date
20/07/2021 11:26
Last modification date
21/07/2021 6:40
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