Inhibition of lymphocyte mediated cytotoxicity by perforin antisense oligonucleotides.

Détails

ID Serval
serval:BIB_80CCB8A48F89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inhibition of lymphocyte mediated cytotoxicity by perforin antisense oligonucleotides.
Périodique
EMBO Journal
Auteur(s)
Acha-Orbea H., Scarpellino L., Hertig S., Dupuis M., Tschopp J.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
1990
Volume
9
Numéro
12
Pages
3815-3819
Langue
anglais
Résumé
The granule/perforin exocytosis model of CTL mediated cytolysis proposes that CTL, upon recognition of the specific targets, release the cytolytic, pore-forming protein perforin into the intercellular space which then mediates the cytotoxic effect. However, direct evidence for the involvement of perforin is still lacking, and indeed, recent results even seem incompatible with the model. To determine directly the role of perforin in CTL cytotoxicity, perforin antisense oligonucleotides were exogenously added during the stimulation of mouse spleen derived T cells and human peripheral blood lymphocytes (PBL), respectively. Perforin protein expression in lymphocytes was reduced by up to 65%, and cytotoxicity of stimulated T cells by as much as 69% (5.7-fold). These results provide the first experimental evidence for a crucial role of perforin in lymphocyte mediated cytotoxicity.
Mots-clé
Animals, Base Sequence, Cell Line, Cells, Cultured, Cytotoxicity, Immunologic/drug effects, Humans, Membrane Glycoproteins, Membrane Proteins/genetics, Membrane Proteins/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Oligonucleotides, Antisense/chemical synthesis, Oligonucleotides, Antisense/metabolism, Perforin, Pore Forming Cytotoxic Proteins, Spleen/immunology, T-Lymphocytes, Cytotoxic/drug effects, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:48
Dernière modification de la notice
20/08/2019 15:41
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