Inhibition of lymphocyte mediated cytotoxicity by perforin antisense oligonucleotides.

Details

Serval ID
serval:BIB_80CCB8A48F89
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inhibition of lymphocyte mediated cytotoxicity by perforin antisense oligonucleotides.
Journal
EMBO Journal
Author(s)
Acha-Orbea H., Scarpellino L., Hertig S., Dupuis M., Tschopp J.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Publication state
Published
Issued date
1990
Volume
9
Number
12
Pages
3815-3819
Language
english
Abstract
The granule/perforin exocytosis model of CTL mediated cytolysis proposes that CTL, upon recognition of the specific targets, release the cytolytic, pore-forming protein perforin into the intercellular space which then mediates the cytotoxic effect. However, direct evidence for the involvement of perforin is still lacking, and indeed, recent results even seem incompatible with the model. To determine directly the role of perforin in CTL cytotoxicity, perforin antisense oligonucleotides were exogenously added during the stimulation of mouse spleen derived T cells and human peripheral blood lymphocytes (PBL), respectively. Perforin protein expression in lymphocytes was reduced by up to 65%, and cytotoxicity of stimulated T cells by as much as 69% (5.7-fold). These results provide the first experimental evidence for a crucial role of perforin in lymphocyte mediated cytotoxicity.
Keywords
Animals, Base Sequence, Cell Line, Cells, Cultured, Cytotoxicity, Immunologic/drug effects, Humans, Membrane Glycoproteins, Membrane Proteins/genetics, Membrane Proteins/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Oligonucleotides, Antisense/chemical synthesis, Oligonucleotides, Antisense/metabolism, Perforin, Pore Forming Cytotoxic Proteins, Spleen/immunology, T-Lymphocytes, Cytotoxic/drug effects, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Create date
24/01/2008 14:48
Last modification date
20/08/2019 14:41
Usage data