Subtractive hybridization techniques to study cellular senescence.

Détails

ID Serval
serval:BIB_7F949D70930E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Subtractive hybridization techniques to study cellular senescence.
Périodique
Methods in molecular biology
Auteur⸱e⸱s
Sternberg M.B., Gepstein S.
ISSN
1064-3745 (Print)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
371
Pages
289-305
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Résumé
Molecular and cellular processes related to the senescence syndrome are determined by programs of differential gene expression. Subtractive cDNA hybridization is a powerful approach to identify and isolate differentially expressed genes in various systems. A highly effective method, termed suppression subtractive hybridization (SSH), has been applied for the generation of subtracted cDNA library of senescing leaves. The method consists of two main stages, the normalization step that equalizes the abundance of cDNAs within the target population and the subtraction step that eliminates the common sequences between the target and the driver populations. The successful generation of library containing high numbered of rare and abundant cDNA clones in senescing plant cells proves the applicability of this method for global identification of differentially expressed genes during cellular senescence.
Mots-clé
Cellular Senescence/genetics, Cloning, Molecular, Gene Library, Nucleic Acid Hybridization, Plant Physiological Phenomena, Plants/genetics
Pubmed
Création de la notice
26/07/2019 17:42
Dernière modification de la notice
21/08/2019 6:35
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