Subtractive hybridization techniques to study cellular senescence.

Details

Serval ID
serval:BIB_7F949D70930E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Subtractive hybridization techniques to study cellular senescence.
Journal
Methods in molecular biology
Author(s)
Sternberg M.B., Gepstein S.
ISSN
1064-3745 (Print)
ISSN-L
1064-3745
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
371
Pages
289-305
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Abstract
Molecular and cellular processes related to the senescence syndrome are determined by programs of differential gene expression. Subtractive cDNA hybridization is a powerful approach to identify and isolate differentially expressed genes in various systems. A highly effective method, termed suppression subtractive hybridization (SSH), has been applied for the generation of subtracted cDNA library of senescing leaves. The method consists of two main stages, the normalization step that equalizes the abundance of cDNAs within the target population and the subtraction step that eliminates the common sequences between the target and the driver populations. The successful generation of library containing high numbered of rare and abundant cDNA clones in senescing plant cells proves the applicability of this method for global identification of differentially expressed genes during cellular senescence.
Keywords
Cellular Senescence/genetics, Cloning, Molecular, Gene Library, Nucleic Acid Hybridization, Plant Physiological Phenomena, Plants/genetics
Pubmed
Create date
26/07/2019 17:42
Last modification date
21/08/2019 6:35
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