Direct and astrocyte-mediated effects of ethanol on brain-derived endothelial cells

Détails

ID Serval
serval:BIB_7EC6530B600F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Direct and astrocyte-mediated effects of ethanol on brain-derived endothelial cells
Périodique
Life Sciences
Auteur⸱e⸱s
Juillerat-Jeanneret  L., Dessous  L. 'E., Eskenasy-Cottier  A. C., Janzer  R. C.
ISSN
0024-3205 (Print)
Statut éditorial
Publié
Date de publication
1995
Volume
56
Numéro
18
Pages
1499-1509
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
The effects of ethanol have been studied in the central nervous system, however there exists only scarce information about the effects of ethanol on endothelial cells forming the blood-brain barrier. As some properties of brain endothelial cells are modulated by underlying astrocytes, the effect of ethanol on cerebral microvasculature might be indirect and mediated by astrocytes. To analyse this question, we added to rat brain-derived endothelial cells (rbEC) in culture either only ethanol (0, 15 and 150 mM) or ethanol conjointly with soluble factors secreted by astrocytes. Alternatively, astrocytes were exposed to ethanol and the medium was added to rbEC. The effects of treatments were evaluated on cell growth and expression of specific proteolytic markers of rbEC. The experiments showed that while the addition of ethanol alone to rbEC increased the expression of gamma-glutamyltranspeptidase and cell growth following an initial toxic effect, the most significant effects were seen when ethanol was added to rbEC together with astrocytic factors or when medium conditioned by astrocytes exposed to ethanol was added to rbEC. In particular, the expression of angiotensin converting enzyme in endothelial cells was dose-dependently increased. These results indicate that the hypertensive and toxic effects of ethanol are mediated by ethanol and soluble factor(s) secreted by astrocytes and dependent on the expression of angiotensin converting enzyme in the brain endothelium. Thus, when evaluating in vitro the effects of toxic substances such as ethanol on the cerebral endothelium, the modulating effect of cells surrounding cerebral vessels must be accounted for
Mots-clé
Angiotensin-Converting Enzyme Inhibitors/pharmacology/Animals/Astrocytes/drug effects/physiology/secretion/Biological Factors/Brain/cytology/Cells,Cultured/Drug Synergism/Endothelium,Vascular/Ethanol/toxicity/Lung/Microcirculation/Peptidyl-Dipeptidase A/metabolism/Rats/Solubility/Time Factors/gamma-Glutamyltransferase
Pubmed
Web of science
Création de la notice
29/01/2008 19:34
Dernière modification de la notice
20/08/2019 15:39
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