Direct and astrocyte-mediated effects of ethanol on brain-derived endothelial cells

Details

Serval ID
serval:BIB_7EC6530B600F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct and astrocyte-mediated effects of ethanol on brain-derived endothelial cells
Journal
Life Sciences
Author(s)
Juillerat-Jeanneret  L., Dessous  L. 'E., Eskenasy-Cottier  A. C., Janzer  R. C.
ISSN
0024-3205 (Print)
Publication state
Published
Issued date
1995
Volume
56
Number
18
Pages
1499-1509
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
The effects of ethanol have been studied in the central nervous system, however there exists only scarce information about the effects of ethanol on endothelial cells forming the blood-brain barrier. As some properties of brain endothelial cells are modulated by underlying astrocytes, the effect of ethanol on cerebral microvasculature might be indirect and mediated by astrocytes. To analyse this question, we added to rat brain-derived endothelial cells (rbEC) in culture either only ethanol (0, 15 and 150 mM) or ethanol conjointly with soluble factors secreted by astrocytes. Alternatively, astrocytes were exposed to ethanol and the medium was added to rbEC. The effects of treatments were evaluated on cell growth and expression of specific proteolytic markers of rbEC. The experiments showed that while the addition of ethanol alone to rbEC increased the expression of gamma-glutamyltranspeptidase and cell growth following an initial toxic effect, the most significant effects were seen when ethanol was added to rbEC together with astrocytic factors or when medium conditioned by astrocytes exposed to ethanol was added to rbEC. In particular, the expression of angiotensin converting enzyme in endothelial cells was dose-dependently increased. These results indicate that the hypertensive and toxic effects of ethanol are mediated by ethanol and soluble factor(s) secreted by astrocytes and dependent on the expression of angiotensin converting enzyme in the brain endothelium. Thus, when evaluating in vitro the effects of toxic substances such as ethanol on the cerebral endothelium, the modulating effect of cells surrounding cerebral vessels must be accounted for
Keywords
Angiotensin-Converting Enzyme Inhibitors/pharmacology/Animals/Astrocytes/drug effects/physiology/secretion/Biological Factors/Brain/cytology/Cells,Cultured/Drug Synergism/Endothelium,Vascular/Ethanol/toxicity/Lung/Microcirculation/Peptidyl-Dipeptidase A/metabolism/Rats/Solubility/Time Factors/gamma-Glutamyltransferase
Pubmed
Web of science
Create date
29/01/2008 18:34
Last modification date
20/08/2019 14:39
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