Identification of new DNA-associated proteins from Waddlia chondrophila.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_7EC1BBAAC2B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of new DNA-associated proteins from Waddlia chondrophila.
Périodique
Scientific reports
Auteur(s)
Barsy M., Herrgott L., Martin V., Pillonel T., Viollier P.H., Greub G.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
20/03/2019
Peer-reviewed
Oui
Volume
9
Numéro
1
Pages
4885
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ <sup>66</sup> ), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/04/2019 7:52
Dernière modification de la notice
30/04/2021 6:12
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