Identification of new DNA-associated proteins from Waddlia chondrophila.

Details

Serval ID
serval:BIB_7EC1BBAAC2B7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of new DNA-associated proteins from Waddlia chondrophila.
Journal
Scientific reports
Author(s)
Barsy M., Herrgott L., Martin V., Pillonel T., Viollier P.H., Greub G.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
20/03/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
4885
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ <sup>66</sup> ), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.
Pubmed
Web of science
Open Access
Yes
Create date
15/04/2019 7:52
Last modification date
01/11/2020 6:23
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