A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.

Détails

Ressource 1Télécharger: Droguerre-2019-A New Tool for In Vivo Study of.pdf (4450.38 [Ko])
Etat: Public
Version: de l'auteur
Licence: CC BY 4.0
ID Serval
serval:BIB_7C1615ED7809
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.
Périodique
Scientific reports
Auteur(s)
Droguerre M., Tsurugizawa T., Duchêne A., Portal B., Guiard B.P., Déglon N., Rouach N., Hamon M., Mouthon F., Ciobanu L., Charvériat M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
04/12/2019
Peer-reviewed
Oui
Volume
9
Numéro
1
Pages
18292
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Astrocytes are glial cells organized in dynamic and structured networks in the brain. These plastic networks, involving key proteins such as connexin 43 (Cx43), are engaged in fine neuronal tuning and have recently been considered as emerging therapeutic targets in central nervous system disorders. We developed and validated a new application of the manganese-enhanced magnetic resonance imaging (MEMRI) technique allowing in vivo investigations of astrocyte-neuron interactions through quantification of brain Cx43 functional activity. The proof of concept has been achieved by quantification of MEMRI signals in brain after either local astrocyte-specific Cx43 knockdown with shRNA or systemic administration of Cx43 blockers. Unilateral hippocampal Cx43 genetical silencing was associated with an ipsilateral local increase of MEMRI signal. Furthermore, Cx43 blockers also enhanced MEMRI signal responses in hippocampus. Altogether, these data reveal the MEMRI technique as a tool for quantitative imaging of in vivo Cx43-dependent function in astrocytes under physiological and pathological conditions.
Mots-clé
Multidisciplinary
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/12/2019 14:09
Dernière modification de la notice
05/01/2020 7:18
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